Multiplex real-time PCR for detection of an epidemic KPC-producing Klebsiella pneumoniae ST258 clone.

نویسندگان

  • Liang Chen
  • Kalyan D Chavda
  • José R Mediavilla
  • Yanan Zhao
  • Henry S Fraimow
  • Stephen G Jenkins
  • Michael H Levi
  • Tao Hong
  • Albert D Rojtman
  • Christine C Ginocchio
  • Robert A Bonomo
  • Barry N Kreiswirth
چکیده

We describe a multiplex real-time PCR assay capable of identifying both the epidemic Klebsiella pneumoniae ST258 clone and bla(KPC) carbapenemase genes in a single reaction. The assay displayed excellent sensitivity (100%) and specificity (100%) for identification of ST258 clone and bla(KPC) in a collection of 75 K. pneumoniae isolates comprising 41 sequence types. Our results suggest that this assay is an effective tool for surveillance of this clone among carbapenem-resistant K. pneumoniae clinical isolates.

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عنوان ژورنال:
  • Antimicrobial agents and chemotherapy

دوره 56 6  شماره 

صفحات  -

تاریخ انتشار 2012